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cx 4945  (TargetMol)


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    Structured Review

    TargetMol cx 4945
    a Immunofluorescence analyses of the cellular localization of endogenous PA28γ and E4F1 in HNSCC cells. DAPI (blue), nucleus; Scale bars, 10 μm. b WCLs derived from HNSCC cells were immunoprecipitated with control IgG, anti-PA28γ, or anti-E4F1 antibody, followed by IB analysis of co-IP products and WCLs. c Schematic representation of the various E4F1 truncations used in the PA28γ-binding assays tested for their capacity to bind PA28γ. The ubiquitin E3 ligase active region is represented by a green box, while the C 2 H 2 and C 2 HC zinc finger motifs are highlighted in yellow and pink, respectively (Left). IB analysis of co-IP products and WCLs derived from HEK293T cells transfected with Flag-PA28γ and Myc-E4F1 truncations (Right). d IB analysis of co-IP products and WCLs derived from PA28γ-rescued HEK293T cells transfected with indicated constructs. e PA28γ-rescued HEK293T cells transfected with the indicated plasmids were stimulated without or with 50 μM TBB for 30 min before harvesting. WCLs were immunoprecipitated with Flag-beads, followed by IB analysis of co-IP products and WCLs. f HSC-3 cells transduced with control or Flag-PA28γ lentiviral vectors and silenced with siNC or siCK2 were stimulated without or with 100ngml –1 EGF for 30 min before harvesting. WCLs were immunoprecipitated with Flag-beads, followed by IB analysis of co-IP products and WCLs. g IB analysis of co-IP products and WCLs derived from PA28γ-rescued UM1 cells treated with 100ngml –1 EGF for the indicated time before harvesting. h, i HSC-3 cells were stimulated with 50 μM TBB ( h ) or 1 μM or 5 <t>μM</t> <t>CX-4945</t> ( i ), and 100ngml –1 EGF before harvesting. WCLs were immunoprecipitated with anti-PA28γ antibody, followed by IB analysis of co-IP products and WCLs. j PA28γ-rescued HSC-3 cells were stimulated without or with 50 μM TBB and 100ngml –1 EGF for 30 min before harvesting. WCLs were immunoprecipitated with Flag-beads, followed by IB analysis of co-IP products and WCLs. k HEK293T cells were transfected with indicated plasmids and treated without or with 100ngml –1 EGF and 50 μM TBB for 30 min before being subjected to GST-PD assays and IB analyses. Source data are provided as a Source Data file.
    Cx 4945, supplied by TargetMol, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cx 4945/product/TargetMol
    Average 93 stars, based on 8 article reviews
    cx 4945 - by Bioz Stars, 2026-04
    93/100 stars

    Images

    1) Product Images from "Phosphorylation of PA28γ by CK2 kinase facilitates HNSCC tumor formation and progression"

    Article Title: Phosphorylation of PA28γ by CK2 kinase facilitates HNSCC tumor formation and progression

    Journal: Nature Communications

    doi: 10.1038/s41467-025-67131-7

    a Immunofluorescence analyses of the cellular localization of endogenous PA28γ and E4F1 in HNSCC cells. DAPI (blue), nucleus; Scale bars, 10 μm. b WCLs derived from HNSCC cells were immunoprecipitated with control IgG, anti-PA28γ, or anti-E4F1 antibody, followed by IB analysis of co-IP products and WCLs. c Schematic representation of the various E4F1 truncations used in the PA28γ-binding assays tested for their capacity to bind PA28γ. The ubiquitin E3 ligase active region is represented by a green box, while the C 2 H 2 and C 2 HC zinc finger motifs are highlighted in yellow and pink, respectively (Left). IB analysis of co-IP products and WCLs derived from HEK293T cells transfected with Flag-PA28γ and Myc-E4F1 truncations (Right). d IB analysis of co-IP products and WCLs derived from PA28γ-rescued HEK293T cells transfected with indicated constructs. e PA28γ-rescued HEK293T cells transfected with the indicated plasmids were stimulated without or with 50 μM TBB for 30 min before harvesting. WCLs were immunoprecipitated with Flag-beads, followed by IB analysis of co-IP products and WCLs. f HSC-3 cells transduced with control or Flag-PA28γ lentiviral vectors and silenced with siNC or siCK2 were stimulated without or with 100ngml –1 EGF for 30 min before harvesting. WCLs were immunoprecipitated with Flag-beads, followed by IB analysis of co-IP products and WCLs. g IB analysis of co-IP products and WCLs derived from PA28γ-rescued UM1 cells treated with 100ngml –1 EGF for the indicated time before harvesting. h, i HSC-3 cells were stimulated with 50 μM TBB ( h ) or 1 μM or 5 μM CX-4945 ( i ), and 100ngml –1 EGF before harvesting. WCLs were immunoprecipitated with anti-PA28γ antibody, followed by IB analysis of co-IP products and WCLs. j PA28γ-rescued HSC-3 cells were stimulated without or with 50 μM TBB and 100ngml –1 EGF for 30 min before harvesting. WCLs were immunoprecipitated with Flag-beads, followed by IB analysis of co-IP products and WCLs. k HEK293T cells were transfected with indicated plasmids and treated without or with 100ngml –1 EGF and 50 μM TBB for 30 min before being subjected to GST-PD assays and IB analyses. Source data are provided as a Source Data file.
    Figure Legend Snippet: a Immunofluorescence analyses of the cellular localization of endogenous PA28γ and E4F1 in HNSCC cells. DAPI (blue), nucleus; Scale bars, 10 μm. b WCLs derived from HNSCC cells were immunoprecipitated with control IgG, anti-PA28γ, or anti-E4F1 antibody, followed by IB analysis of co-IP products and WCLs. c Schematic representation of the various E4F1 truncations used in the PA28γ-binding assays tested for their capacity to bind PA28γ. The ubiquitin E3 ligase active region is represented by a green box, while the C 2 H 2 and C 2 HC zinc finger motifs are highlighted in yellow and pink, respectively (Left). IB analysis of co-IP products and WCLs derived from HEK293T cells transfected with Flag-PA28γ and Myc-E4F1 truncations (Right). d IB analysis of co-IP products and WCLs derived from PA28γ-rescued HEK293T cells transfected with indicated constructs. e PA28γ-rescued HEK293T cells transfected with the indicated plasmids were stimulated without or with 50 μM TBB for 30 min before harvesting. WCLs were immunoprecipitated with Flag-beads, followed by IB analysis of co-IP products and WCLs. f HSC-3 cells transduced with control or Flag-PA28γ lentiviral vectors and silenced with siNC or siCK2 were stimulated without or with 100ngml –1 EGF for 30 min before harvesting. WCLs were immunoprecipitated with Flag-beads, followed by IB analysis of co-IP products and WCLs. g IB analysis of co-IP products and WCLs derived from PA28γ-rescued UM1 cells treated with 100ngml –1 EGF for the indicated time before harvesting. h, i HSC-3 cells were stimulated with 50 μM TBB ( h ) or 1 μM or 5 μM CX-4945 ( i ), and 100ngml –1 EGF before harvesting. WCLs were immunoprecipitated with anti-PA28γ antibody, followed by IB analysis of co-IP products and WCLs. j PA28γ-rescued HSC-3 cells were stimulated without or with 50 μM TBB and 100ngml –1 EGF for 30 min before harvesting. WCLs were immunoprecipitated with Flag-beads, followed by IB analysis of co-IP products and WCLs. k HEK293T cells were transfected with indicated plasmids and treated without or with 100ngml –1 EGF and 50 μM TBB for 30 min before being subjected to GST-PD assays and IB analyses. Source data are provided as a Source Data file.

    Techniques Used: Immunofluorescence, Derivative Assay, Immunoprecipitation, Control, Co-Immunoprecipitation Assay, Binding Assay, Ubiquitin Proteomics, Transfection, Construct, Transduction



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    a Immunofluorescence analyses of the cellular localization of endogenous PA28γ and E4F1 in HNSCC cells. DAPI (blue), nucleus; Scale bars, 10 μm. b WCLs derived from HNSCC cells were immunoprecipitated with control IgG, anti-PA28γ, or anti-E4F1 antibody, followed by IB analysis of co-IP products and WCLs. c Schematic representation of the various E4F1 truncations used in the PA28γ-binding assays tested for their capacity to bind PA28γ. The ubiquitin E3 ligase active region is represented by a green box, while the C 2 H 2 and C 2 HC zinc finger motifs are highlighted in yellow and pink, respectively (Left). IB analysis of co-IP products and WCLs derived from HEK293T cells transfected with Flag-PA28γ and Myc-E4F1 truncations (Right). d IB analysis of co-IP products and WCLs derived from PA28γ-rescued HEK293T cells transfected with indicated constructs. e PA28γ-rescued HEK293T cells transfected with the indicated plasmids were stimulated without or with 50 μM TBB for 30 min before harvesting. WCLs were immunoprecipitated with Flag-beads, followed by IB analysis of co-IP products and WCLs. f HSC-3 cells transduced with control or Flag-PA28γ lentiviral vectors and silenced with siNC or siCK2 were stimulated without or with 100ngml –1 EGF for 30 min before harvesting. WCLs were immunoprecipitated with Flag-beads, followed by IB analysis of co-IP products and WCLs. g IB analysis of co-IP products and WCLs derived from PA28γ-rescued UM1 cells treated with 100ngml –1 EGF for the indicated time before harvesting. h, i HSC-3 cells were stimulated with 50 μM TBB ( h ) or 1 μM or 5 <t>μM</t> <t>CX-4945</t> ( i ), and 100ngml –1 EGF before harvesting. WCLs were immunoprecipitated with anti-PA28γ antibody, followed by IB analysis of co-IP products and WCLs. j PA28γ-rescued HSC-3 cells were stimulated without or with 50 μM TBB and 100ngml –1 EGF for 30 min before harvesting. WCLs were immunoprecipitated with Flag-beads, followed by IB analysis of co-IP products and WCLs. k HEK293T cells were transfected with indicated plasmids and treated without or with 100ngml –1 EGF and 50 μM TBB for 30 min before being subjected to GST-PD assays and IB analyses. Source data are provided as a Source Data file.
    Cx 4945, supplied by TargetMol, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cx  (TargetMol)
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    TargetMol cx
    a Immunofluorescence analyses of the cellular localization of endogenous PA28γ and E4F1 in HNSCC cells. DAPI (blue), nucleus; Scale bars, 10 μm. b WCLs derived from HNSCC cells were immunoprecipitated with control IgG, anti-PA28γ, or anti-E4F1 antibody, followed by IB analysis of co-IP products and WCLs. c Schematic representation of the various E4F1 truncations used in the PA28γ-binding assays tested for their capacity to bind PA28γ. The ubiquitin E3 ligase active region is represented by a green box, while the C 2 H 2 and C 2 HC zinc finger motifs are highlighted in yellow and pink, respectively (Left). IB analysis of co-IP products and WCLs derived from HEK293T cells transfected with Flag-PA28γ and Myc-E4F1 truncations (Right). d IB analysis of co-IP products and WCLs derived from PA28γ-rescued HEK293T cells transfected with indicated constructs. e PA28γ-rescued HEK293T cells transfected with the indicated plasmids were stimulated without or with 50 μM TBB for 30 min before harvesting. WCLs were immunoprecipitated with Flag-beads, followed by IB analysis of co-IP products and WCLs. f HSC-3 cells transduced with control or Flag-PA28γ lentiviral vectors and silenced with siNC or siCK2 were stimulated without or with 100ngml –1 EGF for 30 min before harvesting. WCLs were immunoprecipitated with Flag-beads, followed by IB analysis of co-IP products and WCLs. g IB analysis of co-IP products and WCLs derived from PA28γ-rescued UM1 cells treated with 100ngml –1 EGF for the indicated time before harvesting. h, i HSC-3 cells were stimulated with 50 μM TBB ( h ) or 1 μM or 5 <t>μM</t> <t>CX-4945</t> ( i ), and 100ngml –1 EGF before harvesting. WCLs were immunoprecipitated with anti-PA28γ antibody, followed by IB analysis of co-IP products and WCLs. j PA28γ-rescued HSC-3 cells were stimulated without or with 50 μM TBB and 100ngml –1 EGF for 30 min before harvesting. WCLs were immunoprecipitated with Flag-beads, followed by IB analysis of co-IP products and WCLs. k HEK293T cells were transfected with indicated plasmids and treated without or with 100ngml –1 EGF and 50 μM TBB for 30 min before being subjected to GST-PD assays and IB analyses. Source data are provided as a Source Data file.
    Cx, supplied by TargetMol, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cx/product/TargetMol
    Average 93 stars, based on 1 article reviews
    cx - by Bioz Stars, 2026-04
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    a Immunofluorescence analyses of the cellular localization of endogenous PA28γ and E4F1 in HNSCC cells. DAPI (blue), nucleus; Scale bars, 10 μm. b WCLs derived from HNSCC cells were immunoprecipitated with control IgG, anti-PA28γ, or anti-E4F1 antibody, followed by IB analysis of co-IP products and WCLs. c Schematic representation of the various E4F1 truncations used in the PA28γ-binding assays tested for their capacity to bind PA28γ. The ubiquitin E3 ligase active region is represented by a green box, while the C 2 H 2 and C 2 HC zinc finger motifs are highlighted in yellow and pink, respectively (Left). IB analysis of co-IP products and WCLs derived from HEK293T cells transfected with Flag-PA28γ and Myc-E4F1 truncations (Right). d IB analysis of co-IP products and WCLs derived from PA28γ-rescued HEK293T cells transfected with indicated constructs. e PA28γ-rescued HEK293T cells transfected with the indicated plasmids were stimulated without or with 50 μM TBB for 30 min before harvesting. WCLs were immunoprecipitated with Flag-beads, followed by IB analysis of co-IP products and WCLs. f HSC-3 cells transduced with control or Flag-PA28γ lentiviral vectors and silenced with siNC or siCK2 were stimulated without or with 100ngml –1 EGF for 30 min before harvesting. WCLs were immunoprecipitated with Flag-beads, followed by IB analysis of co-IP products and WCLs. g IB analysis of co-IP products and WCLs derived from PA28γ-rescued UM1 cells treated with 100ngml –1 EGF for the indicated time before harvesting. h, i HSC-3 cells were stimulated with 50 μM TBB ( h ) or 1 μM or 5 μM CX-4945 ( i ), and 100ngml –1 EGF before harvesting. WCLs were immunoprecipitated with anti-PA28γ antibody, followed by IB analysis of co-IP products and WCLs. j PA28γ-rescued HSC-3 cells were stimulated without or with 50 μM TBB and 100ngml –1 EGF for 30 min before harvesting. WCLs were immunoprecipitated with Flag-beads, followed by IB analysis of co-IP products and WCLs. k HEK293T cells were transfected with indicated plasmids and treated without or with 100ngml –1 EGF and 50 μM TBB for 30 min before being subjected to GST-PD assays and IB analyses. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Phosphorylation of PA28γ by CK2 kinase facilitates HNSCC tumor formation and progression

    doi: 10.1038/s41467-025-67131-7

    Figure Lengend Snippet: a Immunofluorescence analyses of the cellular localization of endogenous PA28γ and E4F1 in HNSCC cells. DAPI (blue), nucleus; Scale bars, 10 μm. b WCLs derived from HNSCC cells were immunoprecipitated with control IgG, anti-PA28γ, or anti-E4F1 antibody, followed by IB analysis of co-IP products and WCLs. c Schematic representation of the various E4F1 truncations used in the PA28γ-binding assays tested for their capacity to bind PA28γ. The ubiquitin E3 ligase active region is represented by a green box, while the C 2 H 2 and C 2 HC zinc finger motifs are highlighted in yellow and pink, respectively (Left). IB analysis of co-IP products and WCLs derived from HEK293T cells transfected with Flag-PA28γ and Myc-E4F1 truncations (Right). d IB analysis of co-IP products and WCLs derived from PA28γ-rescued HEK293T cells transfected with indicated constructs. e PA28γ-rescued HEK293T cells transfected with the indicated plasmids were stimulated without or with 50 μM TBB for 30 min before harvesting. WCLs were immunoprecipitated with Flag-beads, followed by IB analysis of co-IP products and WCLs. f HSC-3 cells transduced with control or Flag-PA28γ lentiviral vectors and silenced with siNC or siCK2 were stimulated without or with 100ngml –1 EGF for 30 min before harvesting. WCLs were immunoprecipitated with Flag-beads, followed by IB analysis of co-IP products and WCLs. g IB analysis of co-IP products and WCLs derived from PA28γ-rescued UM1 cells treated with 100ngml –1 EGF for the indicated time before harvesting. h, i HSC-3 cells were stimulated with 50 μM TBB ( h ) or 1 μM or 5 μM CX-4945 ( i ), and 100ngml –1 EGF before harvesting. WCLs were immunoprecipitated with anti-PA28γ antibody, followed by IB analysis of co-IP products and WCLs. j PA28γ-rescued HSC-3 cells were stimulated without or with 50 μM TBB and 100ngml –1 EGF for 30 min before harvesting. WCLs were immunoprecipitated with Flag-beads, followed by IB analysis of co-IP products and WCLs. k HEK293T cells were transfected with indicated plasmids and treated without or with 100ngml –1 EGF and 50 μM TBB for 30 min before being subjected to GST-PD assays and IB analyses. Source data are provided as a Source Data file.

    Article Snippet: EGF (Sigma, SRP3027), TBB (Sigma, T0826), CX-4945 (TargetMol, T2259), CHIR99021 (TargetMol, T22657 ), KN-93 (TargetMol, T2697), D4476 (TargetMol, T2449), CHX (Sigma, C7698), and MG132 (Sigma, M7449) were used at the indicated times and doses.

    Techniques: Immunofluorescence, Derivative Assay, Immunoprecipitation, Control, Co-Immunoprecipitation Assay, Binding Assay, Ubiquitin Proteomics, Transfection, Construct, Transduction